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How Preimplantation Genetic Diagnose (pgd) Is Done | A Health-and-Fitness Article
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How Preimplantation Genetic Diagnos Performed (PGD)
For PGD enbryos need to be generated in vitro. It must go through the ICSI procedure. The embryo are cultured in viro,mbt sport shoes, byopsy is performed on the third day of fertilization and bad cells are removed from embryo. The biopsied cell is subjected to genetic diagnostic testes like FISH or Polymerase Chain Reaction (PCR). The results of genetic analysis are avialable in 12-36 Hrs. Once the results of the genetic analysis are obtained the enbryo which are normal are then trafsfred back to have the healthy baby.
Basic steps of the PGD are:
* Down Regulation
* Ovarian stimulation
* Oocyte Retrieval
* ICSI
* Embryo Culture
* Embryo Biopsy
* Genetic analysis (FISH or PCR)
* Embryo Transfer
How is embyo biopsy performed?
Cleavage stage embryo:
1- A hole drilled in the zona using laser or acid triodes
2- aspiration pipette broght close to the blastomere to be aspirated
3- the blastomere is aspirated with gentle suction
4- single blastomere with the nucleus clearly visible which is subjected to FISH or PCR.
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