Newcastle disease avian influenza rapid diagnostic testing of colloidal gold tray
the use of colloidal gold test paper details the high-tech products, although easy to operate and to determine known, but there are still some small essentials need to know. The following description of some long-term user experience is summed up, as soon as possible for beginners to be familiar with some help.
sample collection
1,
cheap nike air max 90, the diseased chickens can be put into a jar test
the original design, a chicken is a dipstick test. According to China's actual situation, the disease can also feed into a few chickens elution to measure. 5000 sets the general one (only) a chicken coop with only enough, is to find a virus and then redo a general one that is then combined the two will be enough. First, in order to reduce the cost of doing so,
tory burch flip flops, the second is more samples collected, the accuracy will improve. Adopted only a low probability. However, taken on four or five chickens, the probability is greatly increased.
test virus antibody test not the same as usual. Antibody test, the better to be random chicken chicken listless grasp, so as to measure the general situation of antibodies in chickens; and measuring the virus just caught Withered on the line, as long as a few can capture the virus to chickens confirmed this building group have a virus infection. It does not take up tube is all the infected chickens was infected with one or two.
2, can be taken with a cotton swab to the right patient is the key material.
the two cases. First, upon adoption of live chickens. The other is taken in the dead chickens or chicken kill him and then adopted. When the chickens on the mining
when the swab into the cloaca of chicken within 1 to 2 cm, gently turn several times, to as little as possible stick more liquid manure, because the virus in feces is relatively much less .
dead chickens in the body when taken, it can be part of the body adopted a necropsy. First, the trachea, can be directly adopted in the open trachea. Be careful not to adopt endotracheal sputum, because the virus is not necessarily much phlegm,
discount timberland, and can combine with the flow of gold target, is not conducive to judge. Under normal circumstances, there will be a thin layer of tracheal mucosa of the liquid, dip a few in the trachea, the liquid can be coated with a mucous membrane. Do not stick to other things like blood clots. Direction and tracheal swabs have little anyway, usually working smoothly along the direction of some of the trachea. Another point is taken
intestinal duodenum and small intestine around the middle of the yolk pedicle 1 to 2 cm. Cut where the intestine, from 0.5 to 1 mm scuffing intestinal gut, in the lymphoid follicles (swelling, bleeding, ulcers and other lesions with a cotton swab dipped a couple of buttons. Scuffing because the digesta in the intestinal surface The virus is not more than the lesion. not because of the hard surface scratch the surface of the intestine may have the virus released from the intestinal mucosa. swab the general direction taken in the vertical direction with the bowel as well, you can take one or two ,
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on the use of cotton swabs. because a swab per bird to adopt chickens and may be collected from multiple sites, so use a cotton swab several times in the wash from the virus pull off the liquid out of the bottle when the bottle to the head of a cotton swab on a bi squeeze,
black christian louboutin Get rid of memories, especially when pulling into the bottleneck and then turn the squeeze. so you can stay liquid in the bottle as much as possible, and second, less wet then dip a cotton swab in the liquid can be well coated with the new parts of the liquid, to avoid too much with water and then pull out the stick when the stick when the stick is not on the liquid or less liquid situations.
If you think a cotton swab dipped too much trouble was the number of times, you yourself can also select and provide us with the supermarket close to the cotton swab to buy some. need for multi-purpose one. but to pay attention not to use too much cotton swabs, the virus eluate Do not dip less finished or stained too. left too thick liquid is very beneficial to the subject of pop gold that carried out the determination.
3, collected samples do not fused and the occurrence of the tray handling
test high sensitivity, do not get sick too much material. get sick as long as the material is guaranteed to get onto the lesion on the line. is too thick,
coach outlet online store, not only dropping a sample of the subject combined with the gold, but also affect the gold standard on the test strip flow. some of the gold standard test strip on the run could not finish, because the reason the sample is too thick.
If you accidentally get into how to do this?
First, one can not use another eluent bottle of liquid addition to the thick part of the bottle to dilute it. Second, if the sample found in the dropping gold standard is too thick, then a bad run can be immediately added to the liquid bottle is not enabled in the drip holes one to two drops, so that a number of gold standard run smoothly. If there are particular sample of the material can stick it thick with a clean toothpick to remove; if a large number of samples and urate below the drip hole polyester exudate down the gap membrane blocked the other end when you use a cotton swab hole in the bottom of the polyester film drops a few stir gently down to liquid penetration.
test strips to determine
3, take the cover after the sample end cap, shake the bottle so that the virus samples with the eluent full contact. drip, the drip drip bottle with the holes not in direct contact, you can drop drop drop drop complete, continuous drop of 4 to 5 drops.
5, observe the flow of gold standard should pay attention to. quality control Line the C line to the red line, not that this does not work. Whether the test line that is not T line.
test line is the straight line, parallel with the control line. is not running in a curve, but also not the gold standard from the gold standard pad type just ran out of the line when the meniscus. to determine the results of gold standard as far as possible when you finish, the gold standard is useless to go to the top of the absorbent filter paper is to take the good.
Another test line width of about a millimeter, and sometimes not run when the gold standard to show it. In both the upper and lower case gold standard ion, just showing a white about 1 mm wide lines, the situation is still is negative, because the virus just want to be caught in this line, indicating that no virus or virus does not to this line. appears that gold standard run this bad, this is not a plus to add a drop or two gold standard of liquid samples to see the smooth running past, not to do that this result, redo the test strip to be changed.
slightly better than the situation is that in the up and down the red against the background of gold standard ion appears wide for the penny-meter-wide white line there was a narrow red, this is a virus that is positive, but the run was not very good. You can also redo.
If the gold standard because of thick and not run, you can Gold standard not used during the operation of dropping the new liquids, first dilute the flow of second is to add some power, usually as the case may add one to two drops. This action is effective in operation within ten minutes, over twenty-five minutes, not much significance.
user psychological changes
6, test strip from the access to skilled about three stages.
First, the testing phase of the product available at this time Newcastle influenza antigen (or vaccine) to directly test strip can not be used, the sensitivity is. in the process also cognitive test strips can be most skillfully use. This process is very important, many comrades are often reluctant to do this work, take the test strip directly to a user in the field drug test. is often undesirable because of the situation can not cope. Old people always say, may quicken power. Even if you feel a little strip, but also should first learn well and then do the work. colloidal gold products, although the high-tech and easy to use,
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The second stage is the stage of psychological impact. After the first stage, the product no doubt about the quality and sensitivity and can master the method of detection after the test strip. We many veterinarians, especially in dry years do not feel the low level of self-good or feel gay encounter with the test strip where the conflict, the process is more prominent in the psychological. that is recognized within their own experience of avian influenza or Newcastle disease virus test undetectable, or that there is no virus detected a virus disease. that means the results of testing experience with the usual conflict between the judge. with veterinary own words, usually see standards, and returned to see good people, the results of a test do not have the virus. This is not annoying you?
Grossly necropsy laboratory for actually means, accuracy or much worse, is colloidal payment methods such as micro hemagglutination than the laboratory class is even higher sensitivity of the method of magnitude 5 to 9. According to our experience, veterinary avian influenza and Newcastle disease in the judgments on more than one third at least is not correct, there is errors in more than half. and many successful examples where the disease is sometimes not.
how the experience of the old doctor's experience with the new combination to end the conflict is that each user must have a psychological Off. Of course, the level of veterinary itself has also been an essential improvement.
The third stage is the skilled application stage. then advanced equipment and products is also a tool for people. In the course of medical treatment of people play a decisive role . test strip and then senior assistant is a good person. At this stage one can achieve one device. one can always use any test strip to verify or supplement their own judgments, on the other hand I could be the result of the flexibility to deal with test strips . can come from the disease to consider the overall change in the amount of virus particles, taking into account the sensitivity of the product and so on. and then make use of high sensitivity and high specificity of the test paper to separate the identification of the virus, or promotion to the usual breeding ground testing, this much of its meaning when.
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